In the first part of my talk, I will introduce our scalable and modular computational pipeline (MCMICRO) and the Minimum Information about Highly Multiplexed Tissue Imaging (MITI) standard as well as our spatial power analysis framework to improve experimental design strategies. In the second part, I will highlight how we utilized these tools to process data with a focus on myocardial infarction and how we are currently building a translational center for spatial profiling (Translational Spatial Profiling Center - TSPC).