As an electrically active tumor, GBM diffusely colonizes healthy brain tissues, disrupting neurotransmission balance and leading to glioma-related epilepsy (GRE) in up to 50% of patients. Interestingly, GRE is an independent favorable prognostic factor for survival in GBM, highlighting the complex interplay between the tumor and brain function. To develop more effective therapies, it's crucial to understand the glioma microenvironment and tumor-healthy brain crosstalk. Sequential spatial immunofluorescence has allowed us to assess novel therapies holistically, revealing their effects on tumor growth, its microenvironment infiltrate, and communication. Our research is focused on unraveling the effects of extrinsic electrical modulation on the glioma microenvironment, exploring how this influences tumor growth and its impact on both tumoral and healthy tissues.
